How To Make A Glove Box Clean Room

Cleaning your isolator/glovebox periodically reduces the percent of cross-contamination after each use. In essence, an isolation glove box is a scaled-down version of a cleanroom; similar cleanroom cleaning procedures can be used. The cleaning steps below can be used across a variety of glove box system. But, the agents used to clean the enclosure will differ depending on the construction material used for your glove box.

Before starting, review our Cleaning equipment list and Cleaning tips.

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Isolation Glove Box Cleaning Steps

Inside Enclosure

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Just as performing a test inside the glove box chamber, cleaning will be done by using the fixed glove assembly. This will prevent contaminate from drifting into the laboratory.

Start by placing all cleaning supplies inside the main enclosure and closing the door.

1. Cleaning will begin at the top/ceiling of the glove box
2. Using non-abrasive cleaning cloth, wipe left to right with overlapping strokes
3. Fold the wiper into quarters, using a different quarter for each stroke. This ensures no particulate is returned
4. Next move onto cleaning the side walls of your glove box. The back wall left and right wall are next and finish with the front wall
5. An isolation cleaning tool can be used to clean hard to reach areas
   • If you are using a cleaning tool, make sure to change the wipe before starting on a new side
6. Using the same motion, left to right overlapping strokes
7. The floor will be the last surface to clean. Using the same left to right motion
8. Inspect the last wiper, there should be no visible residue

If a cleaning agent has been used for the steps above, a wiper or mop pre-treated serialized with deionized IPA can be used to rinse the cleaning agent.

Outside Enclosure

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If the outside surface is dirty, lightly swab a damp cloth over the unit. Avoid forceful rubbing as this can cause scratches to acrylic and stainless steel surfaces. To dry the acrylic surface use a clean, dry cloth and gently blot the surface.

Following this glove box cleaning procedure will help reduce cross-contamination after each use. Always consult with a laboratory manager before using any type of cleaning agent and wear laboratory safety gear during cleaning.

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Category: HOW